Gardenhire went out to the challenge the play and after a short review, the play was overturned and Aybar was ruled out, ending the inning.
In the ninth, the Twins put two runners on with one out against closer Huston Street, but Brian Dozier grounded into what was ruled a game-ending double play on a check swing. Gardenhire challenged the play but the call was upheld.
Gardenhire has now issued 34 challenges this year with 18 calls overturned.
Rhett Bollinger is a reporter for MLB.com. Read his blog, Bollinger Beat, and follow him on Twitter @RhettBollinger. This story was not subject to the approval of Major League Baseball or its clubs.