Roenicke challenged and the call was overturned, improving the Brewers' record in challenges to 10-5 this season, including five wins in a row.
Segura's stay on base was short. He was erased on a double play when Brewers pitcher Kyle Lohse popped a bunt to Nationals starter Stephen Strasburg.
Adam McCalvy is a reporter for MLB.com. Read his blog, Brew Beat, and follow him on Twitter at @AdamMcCalvy. This story was not subject to the approval of Major League Baseball or its clubs.