The show will be held rain or shine and will be promoted by Live Nation.
This will be the ballpark's second show, following Elton John and Billy Joel's sold-out show in July 2009.
"We pride ourselves on playing at some of the most unique venues in the world," Matthews said. "Nationals Park is the newest and most exciting outdoor venue in the D.C. market, and we are very excited to be the second show to play there."
"We are very excited to have the biggest concert of 2010 as our second summer concert in Nationals Park," Nationals president Stan Kasten said in a statement. "Elton John and Billy Joel gave the D.C. community an unforgettable performance in July 2009 and the Nationals are thrilled to host the 'hometown' Dave Matthews Band in the summer of 2010."
The Dave Matthews Band has sold more than 15 million tickets for its concerts, and has been named the top-drawing American band in the world by Billboard. Pollstar named the group its "Top Act of the Decade" and Spinner dubbed them one of the "Top Artists of the 2000s."
Dave Matthews Band has been nominated for Album of the Year and Best Rock Album at this month's 52nd Annual Grammy Awards.
Formed in Charlottesville, Va., in 1991, the Dave Matthews Band has sold a collective 35 million units (CD and DVD combined). Dave Matthews Band is Carter Beauford (drums), Stefan Lessard (bass), Dave Matthews (vocals, guitar) and Boyd Tinsley (violin). Saxophonist LeRoi Moore, the fifth original founding member, passed away during the band's summer 2008 tour.
Bill Ladson is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.