"I told him on the elevator last night that I didn't care if he woke up feeling like he had someone else's calf, he wasn't playing today," Matheny said.
Berkman left Tuesday night's game in the eighth inning after he felt some tightness in his calf after legging out a triple in the sixth inning. He led off the eighth with a single, then removed himself for a pinch-runner when it became apparent he wasn't going to be able to run the bases effectively.
"That was an important run," Berkman said. "Everyone on the team was faster than me at that point."
On Wednesday morning, Berkman arrived in the visitors' clubhouse with a slight limp. He described the condition of his calf as "Not good," and said he could pinch-hit on Wednesday if necessary, but not run the bases.
"It's a little sore this morning," Berkman said. "It's probably not playable. We'll just have to wait to see how it responds. [Thursday] is an off-day, so that's good."
Berkman, who's batting .375 with six walks, will receive treatment to determine his availability for Friday's home opener at Busch Stadium, and beyond.
"They'll shock it, ice it, do all sorts of hocus pocus," he said.