Gyorko's three-run homer in the second inning sparked a five-run go-ahead rally as Peoria held on to beat Phoenix, 7-6, in the Arizona Fall League.
The Padres' second-round pick last year went 2-for-4 in his second AFL start. San Diego's No. 4 prospect also singled in the eighth and walked in the fourth after collecting three hits Oct. 4 in his debut last week.
"I was a little jumpy in my first at-bat and I wanted to stay back," Gyorko said. "I was able to get a cutter over the plate and put a good swing on it and get it out."
The West Virginia product had a big season this summer at two levels, batting .333 with 25 homers, 114 RBIs and 12 steals between Class A Advanced Lake Elsinore and Double-A San Antonio.
"I think it's always a little difficult to go to the next league," the former second-rounder said of his promotion to the Texas League. "The pitchers are really good up there, you just have to continue to make the adjustments and keep getting better."
Gyorko batted third Monday, ahead of another slugger in Cardinals first baseman Matt Adams, who pumped out 32 homers and 101 RBIs while hitting .300 for Double-A Springfield this year. Adams, who had four hits over his first four games, first put the Javelinas up in the second with a solo homer off Phoenix starter Murphy Smith (Athletics).
"I'd love to hit behind him (Adams) every single at-bat of my career, I'd get good pitches to hit," Gyorko said of the Javelinas' cleanup hitter.
Peoria tacked on a pair of runs to cap the five-run third on RBI hits by San Diego's Jaff Decker and St. Louis' Ryan Jackson. The Javs added another run in the ninth when Decker ripped a leadoff triple and scored on Jackson's single to center.
The Desert Dogs score the bulk of their runs in the second when Rob Segedin hit an RBI double and Segedin scored on Adams' fielding error. Former A's first-round pick Grant Green came home on a sacrifice fly by fellow Oakland prospect Ryan Ortiz for the inning's second run.
Smith was charged with six runs on eight hits over three innings.
San Diego's Nick Schmidt allowed three runs -- two earned -- on two hits and a pair of walks over three frames in the start for Peoria. Seattle's Forrest Snow was sharp in relief, striking out four over two perfect frames.
Danny Wild is an editor and Robert Emrich is a contributor for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.