On Monday, Ramirez was given the day off.
Asked if he had flexibility in his ankle, the Marlins rising star said: "I don't have it right now."
After a day of treatment, manager Fredi Gonzalez is leaning toward having Ramirez back for Tuesday's game with the Mets.
"He went to see the doctor this morning -- just precautionary," Gonzalez said. "He was in the lineup, but he came in a little stiff and we took him out. He's going to get treatment over the course of the day, and I think he will go tomorrow."
Ramirez, the focal point of a young Marlins squad, most likely will rest his ankle over the next few days. He added that the ankle bothered him when he got out of bed on Monday morning.
Speed is a big part of Ramirez's game, so there is no reason to rush his recovery. He stole 51 stolen bases in each of the past two seasons.
To make sure nothing is wrong, Ramirez had an X-ray taken on Monday. With his ankle taped, he didn't take part in any baseball activities.
"I'm day-to-day," he said.
Robert Andino got the start at shortstop on Monday against the Red Sox.
Ramirez was groomed in the Red Sox system, and he was traded to the Marlins after the 2005 season as part of the deal that sent Josh Beckett and Mike Lowell to Boston.
Ramirez said it doesn't bother him to miss a Spring Training game against his former team.
"If it was the regular season, then yeah," he said of wanting to face Boston.
The Marlins play the Red Sox again, on Saturday as part of a split-squad day. Ramirez already is pushing to make the trip to Fort Myers.
"If we were playing a regular season game today, he would be in the lineup," Gonzalez said. "We are always going to err on the side of caution, especially this early in the spring. But if we were playing the Red Sox in the middle of the year, he would be in the lineup."
In 2007, Ramirez was one of the most productive all-around players in the league, batting .332 with 212 hits, 125 runs scored, 29 homers and 81 RBIs.
This spring, he is hitting .300 (3-for-10) in three games.
While Ramirez was resting, center fielder Cameron Maybin, who experienced a tight left hamstring last Friday, did some light jogging for the first time since Monday. Maybin also took batting practice, and he could be in games later in the week.