Valencia struck out swinging on a 3-2 slider from O's reliever Michael Gonzalez and immediately argued with Wendelstedt, as he wasn't happy with the called second strike on the prior pitch.
Valencia then was ejected for the first time in his career and was later replaced at third base by Luke Hughes.
"My emotions got the best of me there," Valencia said. "I probably shouldn't have done it, obviously. I kind of let him know I was displeased, and I shouldn't have done it, and he threw me out. I didn't curse or anything. It was my first and it'll probably be my last."
Gardenhire also came out and exchanged words with Wendelstedt after Valencia was thrown out and ended up getting tossed for the 60th time in his career. It was also the sixth time this season that Gardenhire was ejected, as he was also tossed on Thursday for arguing about an overturned home run hit by Justin Morneau.
"I had to go out and defend a little bit," Gardenhire said. "Hunter and I have actually gotten along really well. It's one of those things where I got a little frustrated. So that's my fault, not Hunter's."
Gardenhire, who has had spats with Wendelstedt in the past, flipped his cap off in his disgust on his way off the field.