With the moves, the Cubs' 40-man roster is now at 34.
Rosario, 26, was 2-1 with 14 saves and a 2.50 ERA combined between Class A Advanced Myrtle Beach, Double-A Tennessee and Iowa. He missed the 2015 season because of an injured right elbow. Originally signed as a non-drafted free agent, Rosario is 27-33 with 17 saves and a 4.78 ERA in 160 career Minor League games.
Mullee, 28, made his Major League debut with the Yankees this past season in two stints. He spent a majority of his season with Triple-A Scranton/Wilkes-Barre, going 4-0 with six saves, a 0.99 ERA and 11 walks. He was placed on the disabled list July 2 with carpal tunnel-like symptoms and then transferred to the 60-day DL on Aug. 13 after undergoing ulnar nerve decompression surgery. He was activated from the 60-day DL on Monday.
Mullee is 13-6 with 14 saves and a 2.00 ERA in 103 career Minor League appearances, all in relief.
Brooks, 26, acquired from the Athletics in February for Chris Coghlan, was on the disabled list because of a hip contusion. Rosscup, 28, was placed on the 60-day disabled list Feb. 26 because of left shoulder inflammation.
Villanueva, 25, suffered a right fibular fracture and was placed on the disabled list April 3. Beeler, 27, was sidelined all season because of right shoulder inflammation.
Acevedo, 26, made six relief appearances with Tennessee this past season but missed a majority of the year because of a torn left ACL in May.
Federowicz, 29, appeared in 17 games for the Cubs, batting .194, and batted .293 for Iowa this season.
Carrie Muskat has covered the Cubs since 1987, and for MLB.com since 2001. She writes a blog, Muskat Ramblings. You can follow her on Twitter @CarrieMuskat and listen to her podcast. This story was not subject to the approval of Major League Baseball or its clubs.