Colon underwent an MRI after the game at New York Presbyterian Hospital. Yankees manager Joe Girardi said he did not know if the injury would require a stint on the disabled list.
"I don't think we'll really know until tomorrow," Girardi said. "He just kind of stretched his lower hamstring, and I think we'll have a better idea what we're dealing with tomorrow."
Cleveland right fielder Shin-Soo Choo grounded Colon's 1-2 offering toward first base, where Mark Teixeira made the stop just behind the bag. Colon came to cover first base, and the Yankees' staff made its way out to the field shortly thereafter to tend to the hurler.
"Bartolo, he's very athletic," Teixeira said. "And so when I caught the ball down the line I was expecting him to be very close to the bag. He gets over there very quickly. And because he wasn't at the bag and limping, I hesitated a little bit and threw a soft throw, but I knew it wasn't good. Because, unfortunately, you do see pitchers get hurt covering first base, just because it's kind of an unnatural thing. They don't do it all the time."
It could potentially be a weighty blow to the Yankees' pitching staff, which also lost reliever Joba Chamberlain for the season earlier this week because of an elbow injury.
Colon (5-3) has turned out to be a nice boost for the rotation, earning his third win Saturday in as many starts. He finished the day with six strikeouts, one walk and two hits scattered across 6 2/3 innings of shutout work.
"Bartolo's throwing the ball like a legitimate No. 1 starter in the American League East," Yankees third baseman Alex Rodriguez said. "His stuff is unbelievable, through the charts. I have him now as like a Cy Young-type guy in 2005. That's the type of stuff he's throwing. We have two aces -- C.C. [Sabathia] and him. Give [Yankees general manager Brian Cashman] a lot of credit. It was the sign of the century."
Colon -- out of baseball since 2009 -- came to Spring Training with the Yankees without many expectations. But he's recaptured the top-level form he showed earlier in his career when he won double-digit games every season from 1998-2005.
"He's been great," Teixeira said. "I had to face him as a Ranger when he was with the Angels. He had a stretch there where he was one of the toughest pitchers in baseball, by far. And this season he's been back to that form. It's been fun to watch."