"He was mad at the call," said manager Brad Ausmus. "From my angle, it looked like it was a little up, so I understand the frustration. A close game and he's the go-ahead run."
It had already been a frustrating weekend for the outfielder, who finished the series 1-for-13 with five strikeouts. Upton had also previously expressed irritation with the strike zone in Houston after an eighth-inning K on Saturday.
It was the first ejection of the season for Upton.
Chris Abshire is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.