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Bour was in the fifth spot on Friday night against the Nationals. But that could be subject to change, depending on the opposing starting pitcher.
The left-handed-hitting Bour hasn't had much experience against lefties, batting .221 in 68 at-bats last year. If he struggles with southpaws, Bour could become a platoon option at first base with Chris Johnson.
Prado doesn't provide the power threat of Bour, who belted 23 homers last year. But Prado is a proven veteran and a strong situational hitter.
Without question, Stanton is the key to maximizing the order. As long as he's in the lineup, the Marlins set up with Dee Gordon leading off, followed by Marcell Ozuna, Christian Yelich and Stanton.
"[Stanton] kind of sets your lineup up in a different way," Mattingly said. "He just kind of brings an instant run at any time. If we can get guys on base, and we put them where they have to pitch to him, that's really where you try to put your lineup together.
"I like Christian at three. I like Ozuna at two, and obviously Dee one."
What follows could be in flux. On Friday, it was Bour, Prado, Adeiny Hechavarria, Jeff Mathis and the pitcher, David Phelps.
When regular catcher J.T. Realmuto is in the lineup, he is a probable choice at seven.
"I'm trying to figure out behind [Stanton]," Mattingly said. "Obviously, we have to get J.B. going, and Martin. If those guys are swinging the bat, then it just puts more pressure on the opposing team."
There is a chance Prado becomes a fixture in the fifth spot once the season gets going because opponents may opt to pitch around Stanton and then bring in a lefty for Bour.
Mattingly then may be forced to pinch-hit.
"If J.B. puts me in a spot where I have to pinch-hit all the time, that puts me in a little bit of a bind late in the game, too."