The national anthem was started by the Royals' mascot, Sluggerrr, and the near capacity crowd all joined in to sing.
The audio system returned during the top of the third inning and was greeted by a loud ovation.
"They told me there'd be no audio and I was just so uncomfortable for two innings," manager Ned Yost said. "I guess you're so used to the music and the announcer. It was a strange feeling and I was really uncomfortable. I was so glad when I heard the organ."
Royals starter Danny Duffy said he wasn't really affected.
"It was different," he said. "It felt like the fans were 10 times more behind us. It was a different look I guess."
Jeffrey Flanagan is a reporter for MLB.com. Follow him on Twitter at @FlannyMLB. This story was not subject to the approval of Major League Baseball or its clubs.