The Indians announced that Brantley is considered day to day for now.
"His back tightened up on him again," Indians manager Terry Francona said. "He wanted to play so bad. We just don't want to do something that prolongs it. We're hopeful he can play tomorrow, but if he doesn't, I'd rather it be a day or two than a week or two. He's battled this on and off all spring, so we just want to get a handle on it."
In 15 Cactus League games this spring, the 27-year-old Brantley hit at a .364 (16-for-44) clip for the Indians. He went 0-for-4 in Cleveland's Opening Day loss to Houston on Monday night at Minute Maid Park. Last season, the outfielder finished third in American League Most Valuable Player voting after batting .327 with 20 homers, 23 stolen bases, 45 doubles, 94 runs and 97 RBIs in 156 games.
With Brantley -- Cleveland's No. 3 hitter -- out of the mix on Wednesday, second baseman Jason Kipnis moved from second to third in the lineup. Shortstop Jose Ramirez slid into the No. 2 spot and utility man Mike Aviles assumed Brantley's place in left field, serving as the No. 9 batter for the Tribe.
Jordan Bastian is a reporter for MLB.com. Read his blog, Major League Bastian, and follow him on Twitter @MLBastian. This story was not subject to the approval of Major League Baseball or its clubs.